Fig. 3.

ITT, Pepck, Glut4, HIF-1α/VEGFA and INSR expression in DIO mice. (A) ITT. Black squares, NCD mice (n = 10); gray circles, HFD mice (n = 10). Values are expressed as mean ± s.e.m. *P < 0.05 vs NCD mice [Student's t-test]. (B) Liver phosphoenolpyruvate carboxykinase (Pepck) mRNA, and Glut4 protein expression in skeletal muscle plasma membranes, as measured by RT-qPCR and WB analysis, respectively, in NCD (n = 10) and HFD (n = 10) mice. A representative WB is shown, together with densitometric analyses of multiple immunoblots (n = 10 animals for each group). *P < 0.05 vs NCD mice [Student's t-test]. (C) INSR mRNA and protein levels in VAT from NCD and HFD mice, as measured by RT-qPCR and WB, respectively. Data from each analysis are representative of 10 mice for each group. *P < 0.05 vs NCD mice [Student's t-test]. (D) HIF-1α and VEGFA protein expression in VAT from NCD (n = 10) and HFD (n = 10) mice, as measured by WB. β-actin, loading control. Representative WBs and mean densitometric analyses of WBs are shown. *P < 0.05 vs NCD [Student's t-test]. (E) INSR mRNA levels in liver and skeletal muscle from NCD (n = 10) and HFD (n = 10) mice, as measured in (C).