Fig. 6.

miR-128 and INSR expression. (A) Determination of endogenous INSR mRNA and protein expression levels in 3T3-L1 adipocytes and HEK-293 cells transfected with either a miR-128 inhibitor or mimic by RT-qPCR and WB, respectively. (B) Endogenous INSR mRNA and protein expression levels were measured as in (A), in 3T3-L1 adipocytes transfected with miR-128 mimic or miR-128 inhibitor and exposed to hypoxia for 48 h before INSR expression was determined. Representative WBs are shown. β-actin, control of protein loading. Densitometric scanning of INSR protein signals are shown in bar graphs. Results in (A,B) are representative of at least 3 independent experiments, each in triplicate: bars are mean ± s.e.m. *P < 0.05 vs normoxic untransfected cells (control, white bar) [Student's t-test].