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. 2020 Sep 18;40(9):BSR20194515. doi: 10.1042/BSR20194515

Figure 1. The preparation of the recombinant anti-TNF-α fusion protein, FVH1-1.

Figure 1

(A) 3D structure of FVH1-1 mimicked by SWISS-MODEL. (B) Identification of FVH1-1 by SDS/PAGE and Western Blot. 1: uninduced, 2: IPTG-induced, 3: supernatant, 4: sediment, M: protein ladder. (C). Identification of the purity of FVH1-1 purified by IDA-Nickel with His-tag using SDS/PAGE. Coomassie Brilliant Blue staining 1: induced BL21(DE3)/FVH1-1; 2: sediment; 3: supernatant; 4–6: washed IBs; 7–8: renaturation protein; 9: further purified protein by IDA-Nickel. (D) Desalting with Äkta. (E) Kinetic analysis of binding of the recombinant anti-TNF-α fusion proteins to hTNF-α by BiaCore.