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. 2020 Aug 31;117(37):22944–22952. doi: 10.1073/pnas.2010545117

Fig. 5.

Fig. 5.

Binding of the BTNL3-BTNL8 heterodimer and CD1b by the BC14.1 TCR. (A) J76 cell lines were stimulated with K562.EV, K562.CD1b, K562.EV.BTNL3+8, or K562.CD1b.BTNL3+8 cells and analyzed for CD69 expression by flow cytometry. One representative experiment of two is shown. (B) Flow cytometry was performed on untransfected 293T cells and 293T cells transfected with the WT BC14.1 TCR or a modified version of the BC14.1 TCR consisting of the DP10.7 Vγ4 combined with the BC14.1 Vδ1 or the BC14.1 Vγ4 combined with the CO3 Vδ1. (C) Flow cytometry was performed on 293T cells transfected with either the WT BC14.1 TCR or the BC14.1 Vδ1 chain paired with a different functional Vγ chain from published CD1c or CD1d-specific γδ TCRs (SI Appendix, Table S1).