Fig. 2.

Conformational changes of TM1 and H8 during β₂AR activation. (A and B) The ¹H-¹³C HSQC spectra of K60^1.59 from TM1 (A) and K333^8.51 from H8 (B). The one-dimensional (1D) slices of corresponding ¹H-¹³C HSQC spectra in H dimension are shown at the bottom. (C–E) The local environments of the two probes are shown by side views in inactive (gray, C) and active conformations (green, D), as well as cytoplasmic views in inactive (gray, E) or active conformations (green, F). The agonist BI-167107 is abbreviated by BI.