Fig. 2.

Silencing of GLO2 does not affect pollen traits. (A and B ) Pollen number and pollen size in untreated S- and l-morph plants and in VIGS-GLO2–treated S- and l-morph plants. Anthers from 10 flowers for each phenotype were used and boxes are as defined in legend to Figure 1. Asterisks indicate significant difference from S-morph flowers by Student’s t test with *P < 0.05, **P < 0.01 and ***P < 0.001. (C) Seed set from crosses between untreated and VIGS-GLO2–treated S-morph plants with either S- or l-morph plants (male × female). Pollen grains from indicated genotypes were used and boxes are as defined above, with n = 12 different crosses per combination. Asterisk indicates the significant difference from the crosses between S- and l-morph plants and between VIGS-GLO2–treated S- and l-morph plants by Student’s t test with ***P < 0.001.