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POLQ, DNA-PK, and BRCA2 can limit IHR. Frequencies of IHR at DSBs (A–C) or nicks (D and E) targeted by transfection of RNPs containing Cas9 or Cas9D10A and the indicated gRNAs (gRNA NC, noncutting control) in HT1080-K2 cells treated with siRNAs to BRCA2, DNA-PKcs, or POLQ, as indicated. IHR frequencies are shown as mean and SD (n ≥ 3); asterisks indicate significant differences from the nontargeting control siRNA, siNT2 (*P < 0.05, **P < 10−3, and ***P < 10−6).
