Fig. 3.

Pigment content and spontaneous activity of Rho^D190N/WT rods. (A) Series of dim-flash responses from Rho^D190N/WT;Gcaps^−/− (green) rods. (B) Normalized ensemble averages of dim-flash responses from Rho^WT/WT;Gcaps^−/− and Rho^D190N/WT;Gcaps^−/− rods (n = 23 and 16 rods, respectively). (C) Dark noise from a Rho^D190N/WT;Gcaps^−/− rod with discrete events marked by stars. (D) Functional pigment content estimated from the probability of failure (Methods) in Rho^WT/WT;Gcaps^−/− (same as in Fig. 2C) and Rho^D190N/WT;Gcaps^−/− rods. Double stars mark statistical significance of P < 0.0001. (E) Cellular rate of spontaneous isomerization events. Each open symbol represents the estimate from an individual rod. There was not a significant difference in cellular event rate between these genotypes. (F) Isoelectric focusing blot showing distinct D190N-Rho (∼35% of total pigment) and WT-Rho (∼65% of total pigment) bands.