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. 2020 Aug 21;19:58–77. doi: 10.1016/j.omtm.2020.08.014

Figure 4.

Figure 4

In Vivo Imaging of AF750-Labeled TBTI3 Retention in the Brain of APPSL Transgenic Mice

3D-reconstruction of the fluorescence signals detected in the head area in WT (A) and APPSL transgenic (B) mice 168 h following i.v. administration of the labeled fluorescent antibody TBTI3a-AF750 using the image of X-ray tomography and fluorescence trans-illumination technology (FLIT). (C) Representative 2D X-ray image of the intracerebral area and extra-cerebral area. Kinetic curves of fluorescent picomoles were detected in the intra-cerebral area (D) or extra-cerebral area (E) of WT and APPSL transgenic mice following i.v. administration of the labeled fluorescent antibody TBTI3a-AF750. The fluorescent molecule was detected by in vivo FLIT imaging procedure (four points of trans-illumination at emission filter: 800 nm; excitation filter: 745 nm) applied at 3, 72, and 168 h following TBTI3a-AF750 i.v. injection. Baseline represents auto-fluorescence levels (zero picomoles of AF750). Each symbol represents the mean (±SEM) of detected picomoles. n = 5 for the WT group and n = 3–5 for the APPSL group. Two-way analysis of variance with repeated factor on time and on ranked transformed values and post hoc Fisher’s test: ∗p < 0.05, ∗∗p < 0.01 for comparison between the WT and the APPSL transgenic mice.