Figure 8.

Effect of TBTI Antibodies on TfR Levels in Brain Endothelial Cells and Neurons

Expression of murine TfR in bEnd.3 cells (A) and in vitro cultured mouse primary cortical neurons (B) following exposure to TBTI2a, TBTI3a, and TBTI6a versus a control IgG1 for 24 h. TfR protein levels from whole cell lysates were normalized to α-tubulin and compared to control IgG1-treated cells, while TfR protein levels from cell surface lysates were normalized to N-cadherin and compared to control IgG1-treated cells. In both whole cell and surface cell lysates, protein levels were assessed by western blot. Bar graphs show means of each group (±SD); p values were obtained by one-way ANOVA with Dunnet’s multiple comparison test versus Ctrl IgG1 groups. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ns p > 0.05.