Table 2.
Kinetic Constants of the TBTIs Mutated in Their Anti-TfR CDRs
| Protein Name | KD (nM) | Kon 104 (1/Ms) | Koff 10−4 (1/s) | Entry | Name |
|---|---|---|---|---|---|
| Anti-mTfRC 8D3 | 0.54 | 7.13 | 0.39 | 1 | |
| TBTI_13C3x8D3_mIgG1 | 2.83 | 3.53 | 0.99 | 2 | TBTI1 |
| TBTI_8D3x13C3_mIgG1 | 0.4 | 7.44 | 0.29 | 3 | TBTI2 |
| TBTI_13C3x8D3 LC_N31A_mIgG1 | 2.18 | 2.5 | 0.54 | 4 | |
| TBTI_13C3x8D3 LC_G68I_mIgG1 | 3.84 | 2.72 | 1.05 | 5 | |
| TBTI_13C3x8D3 LC_N93A_mIgG1 | 4.16 | 2.71 | 1.12 | 6 | |
| TBTI_13C3x8D3 LC_Y49A_mIgG1 | 4.46 | 2.21 | 0.99 | 7 | |
| TBTI_13C3x8D3 LC_Y92A_mIgG1 | 6.88 | 2.17 | 1.49 | 8 | TBTI3 |
| TBTI_13C3x8D3 HC_T100A_mIgG1 | 3.83 | 6.08 | 2.33 | 9 | |
| TBTI_13C3x8D3 HC_Y53A_mIgG1 | 4.25 | 2.92 | 1.24 | 10 | |
| TBTI_13C3x8D3 HC_N31A_mIgG1 | 6.61 | 2.4 | 1.59 | 11 | |
| TBTI_13C3x8D3 HC_S101A_mIgG1 | 8.96 | 2.88 | 2.58 | 12 | TBTI4 |
| TBTI_13C3x8D3 HC_Y52A_mIgG1 | 17.9 | 2.92 | 5.21 | 13 | |
| TBTI_13C3x8D3_HC_Y52A/LC_Y92A_mIgG1 | 29.2 | 2.52 | 7.34 | 14 | TBTI5 |
| TBTI_13C3x8D3_HC_S101A/LC_Y92A_mIgG1 | 6.94 | 5.14 | 3.57 | 15 | |
| TBTI_13C3x8D3_HC_N31A/LC_Y92A_mIgG1 | 8.9 | 6.21 | 5.53 | 16 | |
| TBTI_13C3x8D3_HC_Y52A/LC_Y49A_mIgG1 | 0.86 | 79.3 | 6.89 | 17 | |
| TBTI_13C3x8D3_HC_Y52A-S101A/LC_Y49A-Y92A-mIgG1 | 19.64 | 3.26 | 11.1 | 18 | TBTI6 |
The affinity of TBTI antibodies was calculated from kinetic measurements against mouse transferrin receptor protein by BLI. The bold formatting indicates the specific mutations.