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. 2020 Aug 27;117(37):22992–23000. doi: 10.1073/pnas.2003621117

Table 1.

Association constants for the binding of glycoengineered 3F6-hIgG1 to ligands measured by ELISA

3F6-hIgG1CHO
Ligand Ka, M−1 WT Gal deSia G2F
SpAKKAA 1010 2.54 ± 0.46 2.74 ± 0.23 2.85 ± 0.33 1.93 ± 0.13
Human C1q 106 4.91 ± 0.24 21.7 ± 1.71 5.50 ± 0.15 51.1 ± 6.57
Mouse C1q 106 8.87 ± 0.59 ND ND ND
3F6-hIgG1HEK
Ligand Ka (M−1) WT deGal deSia G0F G1F G2F
SpAKKAA 1010 2.44 ± 0.52 2.86 ± 0.33 2.90 ± 0.32 2.34 ± 0.13 2.24 ± 0.16 1.93 ± 0.16
Human C1q 106 18.7 ± 4.0 4.0 ± 0.70 33.7 ± 3.74 6.94 ± 0.39 38.3 ± 3.58 55.0 ± 6.03
Mouse C1q 106 60.8 ± 7.44 ND ND ND ND ND

WT, wild-type; Gal, galactosylated; deSia, desialylated; ND, not determined; deGal, degalactosylated.