Table 2.

Association constants for the binding of 3F6-hIgG1_CHO or 3F6-hIgG1_HEK variants to ligands measured by ELISA

Ligand	Ka, M−1	WT 3F6-hIgG1CHO	3F6-hIgG1HEK
			WT	KA	LALA	afu
SpAKKAA	1010	2.54 ± 0.46	2.44 ± 0.52	2.85 ± 0.26	2.94 ± 032	2.50 ± 0.25
Human
C1q	106	4.91 ± 0.24	18.7 ± 4.0	1.37 ± 0.62	2.04 ± 1.18	5.97 ± 0.31
FcγRIA	108	7.66 ± 1.35	7.88 ± 1.51	7.86 ± 1.89	0.026 ± 0.086	5.83 ± 4.15
FcγRIIA (R131)	106	2.36 ± 0.39	1.53 ± 0.18	1.81 ± 0.18	0.99 ± 0.11	1.82 ± 0.25
FcγRIIA (H131)	106	1.75 ± 0.30	1.13 ± 0.16	1.23 ± 0.28	0.46 ± 0.029	1.51 ± 0.46
FcγRIIB	105	16.5 ± 1.92	9.25 ± 2.23	11.8 ± 1.55	8.38 ± 2.45	1.79 ± 0.88
FcγRIIIA (V158)	107	2.75 ± 0.50	2.31 ± 0.44	1.40 ± 0.12	0.26 ± 0.14	5.63 ± 0.94
FcγRIIIA (F158)	106	3.09 ± 0.33	1.97 ± 0.25	1.51 ± 0.34	0.85 ± 0.18	9.20 ± 0.14
Mouse
C1q	106	8.87 ± 0.59	60.8 ± 7.44	1.34 ± 0.30	2.91 ± 0.12	2.79 ± 0.16
FcγRI	108	1.77 ± 0.44	1.84 ± 0.48	3.97 ± 0.34	0.021 ± 0.078	0.92 ± 0.094
FcγRIIB	106	1.53 ± 0.26	1.61 ± 0.21	3.35 ± 0.48	1.60 ± 0.18	1.62 ± 0.12
FcγRIII	106	1.49 ± 0.16	1.69 ± 0.19	2.96 ± 0.40	1.72 ± 0.21	2.30 ± 0.34
FcγRIV	108	1.15 ± 0.20	1.35 ± 0.43	2.66 ± 0.88	0.09 ± 0.019	3.19 ± 0.38

WT, wild-type; KA, K322A substitution; LALA, L233A/L234A substitutions; afu, afucosylated.