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. 2020 Sep 1;117(37):22920–22931. doi: 10.1073/pnas.2004489117

Fig. 3.

Fig. 3.

Authentic human VRC01-class naive B cell BCRs can be primed by eOD-GT8 60mer and recruited to GCs at rare physiological precursor frequencies. (A) Frequency of splenic BGC cells in mice immunized with eOD-GT8 60mer, or the eOD-GT8-KO variant, when starting from the indicated HuGL18 B cell precursor frequency (PF). Day 8 splenocytes were analyzed and BGC cells were gated as SSL/B220+/CD4−/CD38−/GL7+ and plotted as percent of total B cells (B220+ cells). (B) Representative flow cytometric plots of HuGL18 B cells in GCs utilizing allotype mark. Cells are gated on GCs as indicated in A. (C) Quantitation of HuGL18 B cells in GCs as indicated in B. Experiments are pooled between two experiments (circles and triangles are separate experiments). A total of 25 mice were examined. (D) Representative histological analysis of individual GCs for HuGL18 B cells in individual GCs as indicated in B. Dotted blue line represents starting affinity. (E) Kinetic experiment showing HuGL18 B cell competitiveness within GCs over time. All data are shown pooled from multiple independent experiments. *P < 0.05. n = 3. n = 3 to 4 mice per experiment.