Fig. 7.
Precursor frequency and affinity are interdependent in determining competitive success for B cells possessing authentic human naive VCR01-class BCRs. (A) Frequency of HuGL B cells in GCs d8 postimmunization following immunization with eOD-GT8 60mer or eOD-GT8KO 60mer. BGC cells gated as SSL/B220+/CD4−/CD38−/GL7+/CD45.1−/CD45.2+. For comparison purposes between mouse strains, HuGL18 plots (Fig. 3) are reshown here. (B) Quantitation of HuGL B cells in GCs as shown in A. All available data are shown. Data were pooled from multiple independent experiments. Dashed line at 1% is provided as visual aide only for the reader to denote responses greater or less than 1%. (C) Statistical comparisons of HuGL GC B cells over time. Kinetics data shown in C are the average of all data shown in Figs. 3E and 6E. Mice were immunized with eOD-GT8 containing HuGL17 or HuGL18 B cells at a precursor frequency of 1 in 106 B cells.*P < 0.05 ***P < 0.001, ****P < 0.0001. n.s., not significant; n = 3, n = 3 to 4 mice per experiment for A and B. n = 4 to 5 for HuGL17 and HuGL18 each, n = 3 to 7 mice per time point for C.