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. 2020 Sep 18;19:1533033820960748. doi: 10.1177/1533033820960748

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — Overexpression of pre-miR-100 reduces phospho-STAT3 and increases p21 (WAF1). (A) Immunoblot showing a reduction in phospho-STAT3 when GBM TICs are transfected with pre-miR-100, siErbB3, siSMRT, or Sm5 (SMARCA5). This pattern is not observed following transfection with the controls. The boxes are included to make the blot easier to interpret. Total STAT3 (T-STAT3) shows no change in total protein level. (B) Quantification of the protein levels in panel (A) shows a reduction 60%–90% of phospho-STAT3 except for the control and siErbB3. (C) p21 protein levels are elevated in response to miR-100-5p overexpression or SMRT silencing. (D) Quantification of the protein levels in panel (C) shows elevated p21 levels compared to the control by 20%–300%. Asterisk denotes statistical significance of p < 0.05. Backgrounds of some images were modified for clarity.