Table 1.
Oligonucleotide primers used for the study of gene expression of selected targets by quantitative real-time PCR.
| Target | Primer sequence (5′–3′) | Annealing temperature, °C | PCR product size, bp | GenBank accession No. (NCBI Reference Sequence) |
|---|---|---|---|---|
| GAPDH | F: ACTTTGGCATTGTGGAGGGT R: GGACGCTGGGATGATGTTCT |
59.5 | 131 | NM_204305.1 |
| ACTB | F: CACAGATCATGTTTGAGACCTT R: CATCACAATACCAGTGGTACG |
60 | 101 | NM_205518.1 |
| PPIA | F: CCTGCTTCCACCGGATCAT R: CCGTTGTGGCGCGTAAA |
60 | 64 | NM_001166326.1 |
| CAT | F: ACCAAGTACTGCAAGGCGAA R: TGAGGGTTCCTCTTCTGGCT |
60 | 245 | NM_001031215 |
| SOD1 | F: AGGGGGTCATCCACTTCC R: CCCATTTGTGTTGTCTCCAA |
60 | 122 | NM_205064.1 |
| GPX2 | F: GAGCCCAACTTCACCCTGTT R: CTTCAGGTAGGCGAAGACGG |
62 | 75 | NM_001277854.1 |
| HMOX1 | F: ACACCCGCTATTTGGGAGAC R: GAACTTGGTGGCGTTGGAGA |
62 | 134 | NM_205344.1 |
| NQO1 | F: GAGCGAAGTTCAGCCCAGT R: ATGGCGTGGTTGAAAGAGGT |
60.5 | 150 | NM_001277619.1 |
| Nrf2 | F: AGACGCTTTCTTCAGGGGTAG R: AAAAACTTCACGCCTTGCCC |
60 | 285 | NM_205117.1 |
| Keap1 | F: GGTTACGATGGGACGGATCA R: CACGTAGATCTTGCCCTGGT |
62 | 135 | XM_025145847.1 |
| NF-κB1 | F: GAAGGAATCGTACCGGGAACA R: CTCAGAGGGCCTTGTGACAGTAA |
59 | 131 | NM_205134.1 |
| TLR2B | F: CTTGGAGATCAGAGTTTGGA R: ATTTGGGAATTTGAGTGCTG |
62 | 238 | NM_001161650.1 |
| TLR4 | F: GTCTCTCCTTCCTTACCTGCTGTTC R: AGGAGGAGAAAGACAGGGTAGGTG |
64.5 | 187 | NM_001030693.1 |
| HSP70 | F: ATGCTAATGGTATCCTGAACG R: TCCTCTGCTTTGTATTTCTCTG |
60 | 145 | NM_001006685.1 |
| HSP90 | F: CACGATCGCACTCTGACCAT R: CTGTCACCTTCTCCGCAACA |
60 | 196 | NM_001109785.1 |
F = forward; R = reverse; GAPDH = glyceraldehyde 3-phosphate dehydrogenase; ACTB = actin, beta; PPIA = peptidylprolyl isomerase A; CAT = catalase; SOD1 = superoxide dismutase 1; GPX2 = glutathione peroxidase 2; HMOX1 = heme oxygenase 1; NQO1 = NAD(P)H quinone dehydrogenase 1; Nrf2 = nuclear factor (erythroid-derived 2)-like 2; Keap1 = kelch like ECH associated protein 1; NF-κB1 = nuclear factor kappa B subunit 1; TLR2B = Toll-like receptor 2 family member B; TLR4 = Toll-like receptor 4; HSP70 = heat shock 70 kDa protein 2; HSP90 = heat shock protein 90 alpha family class A member 1.