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. 2020 Aug 10;39(18):e103932. doi: 10.15252/embj.2019103932

Figure 4. Onco‐RNF43/TP53 KO organoids display an oncogenic transcriptional profile that drives self‐renewal and niche‐independent growth.

Figure 4

  1. Bright‐field microscopy images of WT, TP53KO, and onco‐RNF43/TP53KO human colon organoid lines grown in medium with high Wnt/Rspo (20% conditioned medium (CM)) or without Wnt/Rspo (20, 2 or 0.2% CM). Images were taken 6 days after splitting. Scale bars represent 1,000 μm. Non‐cystic, non‐proliferative organoids are indicated with red asterisks.
  2. Bar plot showing the enrichment scores of significantly enriched MSigDB hallmark gene sets in onco‐RNF43/TP53KO compared to TP53KO organoids (FDR < 0.05).
  3. Bright‐field microscopy and fluorescence microscopy pictures of WT, TP53KO, and onco‐RNF43/TP53KO human colon organoid lines grown in two different media and transduced with the TOP‐GFP reporter. Images were taken 6 days after splitting. Scale bars represent 1,000 μm.
  4. Gene Set Enrichment Analysis of onco‐RNF43/TP53KO compared to TP53KO organoids in medium without Wnt/low Rspo (0.2%). Significantly changed intestinal cell‐type gene sets from Haber et al (2017) are shown (FDR < 0.05).