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. Author manuscript; available in PMC: 2020 Sep 21.
Published in final edited form as: Biochemistry. 2020 Aug 6;59(32):2922–2933. doi: 10.1021/acs.biochem.0c00442

Figure 8. proMMP-9/TIMP-1 complexes bind with greater affinity to LRP1 than proMMP-9 alone.

Figure 8.

Full length LRP1 was immobilized on a CM5 sensor chip using an amine-reactive coupling process. (A) proMMP-9 was injected over the chip in increasing concentrations of ligand (11.1, 33.3, 100, and 300 nM). (B) proMMP-9/TIMP-1 complexes were injected over the chip in increasing concentrations of ligand (3.7, 11.1, 33.3, 100, and 300 nM). Fits of the experimental data (black lines) to a bivalent binding model are shown as blue lines. The data shown is a representative experiment from two independent experiments that were performed.