Table 2.
List of the primers used for qRT-PCR
| Genes | 5′/3′ forward primer | 5′/3′ reverse primer | Access number |
|---|---|---|---|
| Reference gene | |||
| ef1a | GCACGCTCTGCTGGCCTTT | GCGCTCAATCTTCCATCCC | AB075952 |
| Liver metabolism | |||
| gck | GGGTGGTAGGATTTGGTGTG | TGCTGACACAAGGCATCTTC | XM003451020 |
| pfklr | GACGAGCGAGTGGAGAAAAC | TGTCTTGATCCGAGGGAATC | XM003447353 |
| pklr | AGGTACAGGTCACCCGTCAG | CATGTCGCCAGACTTGAAGA | XM005472622 |
| g6pca1 | AGCGTTAAGGCAACTGGAGA | AAAAGCTAACAAGGCCAGCA | XM003448671 |
| g6pca2 | CTTCTTCCCCCTTTGGTTTC | AGACTCCTGCAGCTCCCATA | XM013273429 |
| pck1 | AAGCTTTTGACTGGCAGCAT | TGCTCAGCCAGTGAGAGAGA | XM003448375 |
| pck2 | TACGTCTTGAGCTCCCGTCT | CCTCCTGGATGATGCAAGTT | XM019354843 |
| fasn | AACCTGCTTCTCAAGCCAAA | CGTCACCCCTTGTTCTTTGT | XM013276809 |
| g6pd | GTCACCTCAACCGGGAAGTA | TGGCTGAGGACACCTCTCTT | XM013275693 |
| asat | GCTTCCTTGGTGACTTGGAA | CCAGGCATCTTTCTCCAGAC | XM003451918 |
| alat | CACGGTGAAGAAGGTGGAGT | GCAGTTCAGGGTAGGAGCAG | XM005476466 |
| gdh | CGAGCGAGACTCCAACTACC | TGGCTGTTCTCATGATTTGC | XM003457465 |
| Muscle metabolism | |||
| glut4 | GAGGATGGACATGGAGAGGA | CAGGAAAAGCGAGACTACCG | JN900493 |
| hk1 | CGTCGCTTAGTCCCAGACTC | TGACTGTAGCGTCCTTGTGG | XM019360229 |
| hk2 | CAGAGGGGAATTCGATTTGA | CCCACTCGACATTGACACAC | XM003448615 |
| pfkma | AGGACCTCCAACCAACTGTG | TTTTCTCCTCCATCCACCAG | XM019349871 |
| pfkmb | TTTGTGCATGAGGGTTACCA | CACCTCCAATCACACACAGG | XM003441476 |
| pkma | TGACTGCTTCCTGGTCTGTG | CAGTGAAAGCTGGCAAATGA | XM005447626 |
a
From Yang et al.(40).