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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2020 Jul 23;40(9):e240–e255. doi: 10.1161/ATVBAHA.120.314935

Figure 2. Kir channels are impaired in endothelial cells of obese mice.

Figure 2.

Representative patch clamp recordings of IKir in endothelial cells (ECs) from A) lean and B) obese mouse mesenteric arteries. Inset: bright field (BF) and CD31 fluorescence image of a freshly isolated EC. Scale bar=5 μm. C) Group data of Kir current density (CD) at −100 mV (*p <0.05). Representative recordings of IKir in ECs from D) lean and E) obese mice before (Static) and after flow (Shear). D: A picture of the parallel plate flow chamber. Insets show the shear-induced increase in IKir for respective recordings. Shear-induced CD for each cell tested from F) lean and G) obese mice normalized to static CD (n = 12 cells from 6 lean mice and 9 cells from 4 obese mice, *p<0.05). H) Group data comparing shear-induced increases in EC IKir (*p<0.05).