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. Author manuscript; available in PMC: 2020 Sep 21.
Published in final edited form as: Lab Invest. 2009 Mar 30;89(6):676–694. doi: 10.1038/labinvest.2009.27

Figure 3.

Figure 3

Anti- or pro-apoptosis by HL-60 transformants. (a) Amino acid sequences of the wild-type and mutant RP S19 derived from the constructs. (b) Transcriptions of HA-labeled RP S19 and native RP S19 were detected in Mock-, RPS19-, and Q137N-HL-60 by semi-quantitative RT-PCR. (c) HA-labeled and native RP S19 productions in each of the HL-60 transformants were analyzed by western blot. (d) The monocyte chemotactic potencies of supernatants of apoptotic Mock-, RPS19-, and Q137N-HL-60 at 24 h after manganese (II) loading were compared with simultaneous presence of control PBS (white column), anti-RP S19 rabbit IgG (black column), and C5aRA (hatched column) in chemotaxis chamber, respectively. (e and f) Apoptotic ratios were calculated by cell cycle analysis using Mock- (white column), RPS19- (black column), and Q137N-HL-60 (hatched column) at each time point after the apoptotic stimulations. Data are mean values±s.d. (*P<0.05, **P<0.01).