Skip to main content
. Author manuscript; available in PMC: 2020 Sep 21.
Published in final edited form as: Lab Invest. 2009 Mar 30;89(6):676–694. doi: 10.1038/labinvest.2009.27

Figure 6.

Figure 6

RGS3 in neutrophils. (a) The C5aR on neutrophils (106 cells/ml) was competitively ligated by 10−7 M 125I-labeled peptide mimicking the second binding motif of C5a (YSFKDMQLGR) in a simultaneous presence of various concentrations of the unlabeled C5a-mimicking peptide (the dotted line with open circles) or of a peptide mimicking the binding motif with the C-terminus of C5a/RPS19 (YSFKDMQLDRIAGVAAANKKH) (the plain line with closed circles). After incubation for 60 min on ice, the radioactivity of 125I-C5a peptide bound to the cells was measured for 2 min in a gamma counter. Data are mean values±s.d. (b) Transcriptions of RGS1, RGS3, RGS14, and RGS18 were detected in human neutrophils at 4 h after stimulation with chemoattractants by semi-quantitative RT-PCR.