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. Author manuscript; available in PMC: 2020 Sep 21.
Published in final edited form as: Lab Invest. 2009 Mar 30;89(6):676–694. doi: 10.1038/labinvest.2009.27

Figure 9.

Figure 9

Anti-apoptosis by RGS3 knock down HL-60. (a) RGS3 production was detected in constitutively expressed control shRNA- (RGS3cont-) and RGS3 shRNA- (RGS3low-) HL-60 by Western blot. (b) The constitutive active GPCR-mediated ERK signals were detected in RGS3low Mock-, and constitutively expressed RGS3- (RGS3-) HL-60 by western blot. (c and d) Apoptotic ratios were calculated by cell cycle analysis using RGS3cont- and RGS3low-HL-60 at each time point after manganese (II) loading. Representative cell cycle result at 6 h in RGS3low-HL-60 (thick line) compared with RGS3cont-HL-60 (thin line). Data are mean values±s.d. (*P<0.05, **P<0.01).