Table 2.

Comparison of drug testing results on 2D and 3D cultures.

Cell Cultures	Drugs	2D	3D
HCT-116 wt	5-FU, oxaliplatin, irinotecan, melphalan	equally and highly sensitive to 5-FU, oxaliplatin, irinotecan and melphalan	resistant or almost totally resistant to 4 standard drugs
HCT-116 wt/GFP
NHEK	gefitinib	antiviral activity in concentrations too high for in vivo applications	gefitinib at concentration 0.5 µM was sufficient to induce meaningful reduction of replication and spreading of virus
SW1353	DXR, CIS, CQ	cell viability in 2D cultures were lower than in 3D cell cultures	cell viability in spheroid cultures were higher than in 2D cell cultures
	SAL	similar results for monolayers and 3D cell cultures