Skip to main content
. 2020 Aug 28;21(17):6250. doi: 10.3390/ijms21176250

Figure 8.

Figure 8

Overexpression of DoRWA3 increased the acetylation level of polysaccharides in A. thaliana. (A) The pCAMBIA1302 vector used for A. thaliana transformation. (B) Semi–quantitative PCR of the DoRWA3 gene in wild type (WT) and three overexpression (OE) transgenic (35S::DoRWA3) lines: OE1, OE2 and OE3. (C) Phenotype of the WT and three OE transgenic lines. (D) qRT-PCR analysis of DoRWA3 in the WT and three OE transgenic lines. (E) The content of released acetic acid in different organs of the WT and three OE transgenic lines. The transcript level of DoRWA3 in OE1 was set as 1. FW, fresh weight; DW, dry weight. Data bars represent the mean ± SD (n = 3). * and ** indicate p < 0.05 and p < 0.01 between the WT and OE transgenic lines according to DMRT.