Figure 2.

Protein expression profiles of several cell markers in LESCs obtained from explants and gold standard cultures treated with FBS or two different human sera: (A) Double immunostaining for K14 (green) and K12 (red) cytokeratins; (B) Immunostaining for p63α; (C) Percentage of K14 and K12 positive cells; (D) Percentage of p63α bright cells. *** Indicates significant differences between the gold standard culture and all the other treatments (p < 0.001), and ^φ between FBS and the indicated treatment (p < 0.05). Scale bar for fluorescence images, 100 micrometers.