Table 4.

Characteristics of each cluster of the hierarchical 12-cluster analysis. For each cluster, variables of cultured cells (expression of several proteins (ICC) or mRNA (PCR)), as well as the content of the human sera, in growth factors and signaling molecules are shown.

	s-PRGF1	s-PRGF2	HS1	HS2
Cornea 1	Cluster 1	Cluster 5	Cluster 8	Cluster 11
Variables of cultured cells	− ICC ΔNp63α	+++ ICC ΔNp63α	+++ PCR Ki67	− PCR K14
	−−− PCR ABCG2	++ Size 3	+ PCR N-Cadherin, ΔNp63α	−− PCR ABCG2, K12
		−− PCR N-Cadherin, Size 2	−−− PCR K12
		−−− PCR Ki67
Content of the sera in GFs	+++ PDGF	+++ TGF	+++ GDNF, SP	+++ HGF, FGFb
	++ VEGF	++ IGF-1	++ NGF	++ KGF, EGF
	− SP	−− FN, VEGF	−−− TNF	−− NGF
	−− EGF, GDNF
	−−− IGF-1
Cornea 2	Cluster 2	Cluster 6	Cluster 9	Cluster 12
Variables of cultured cells	+ PCR K12	+++ Size 1	+++ PCR Ki67	+++ PCR K14, Size 3
	−−− PCR N-Cadherin	++ PCR K14, ABCG2	++ PCR K14, ABCG2	++ PCR ABCG2
		+ PCR K12	− Size1	+ PCR K12
		−− PCR Ki67		−−−Size 2
Content of the sera in GFs	++ PDGF	++ TGF-β, IGF-1	+++ GDNF, SP	+++ HGF, FGFb
	− EGF, GDNF	−− FN, VEGF	++ NGF	++ KGF, EGF
	−− IGF-1		−−− TNF	−− NGF
Cornea 3	Cluster 3	Cluster 7	Cluster 10	Cluster 10
Variables of cultured cells	+++ PCR K12	+++ PCR N-Cadherin	− PCR K12	− PCR K12
	++ ICC ΔNp63α, Size 2	−−− PCR ΔNp63α	−−− PCR K14, ΔNp63α	−−− PCR K14, ΔNp63α
	− Size 3
Content of the sera in GFs	++ PDGF	++ TGF-β, IGF-1	+ FGFb	+ FGFb
	− IGF-1	−− FN, VEGF

Positive (+) and negative (−) statistically significant differences between cluster mean and global mean: + (p < 0.05), ++ (p < 0.01) and +++ (p < 0.001); − (p < 0.05), −− (p < 0.01) and −−− (p < 0.001). Cluster 4 has been excluded from the table because it only contains two samples corresponding to two different treatments. GF: growth factors; Size 1: cell size < 12 µm; Size 2: 12–20 µm; Size 3: cell size > 20 µm.