Figure 2.

Filopodia-like tentacles in salt-induced C. merolae cells. (A) Fraction of cells in suspension culture with tentacles before (0 %, N_tentacles = 0, N_total = 176, n = 4) and after addition of 50 mM NaCl (39.6 ± 8.7 %, N_tentacles = 69, N_total = 178, n = 4). (B–D) Time series of C. merolae cells growing filopodia-like projections (arrowheads) from bulbs (arrows) upon induction with 50 mM sodium chloride imaged in phase-contrast microscopy. (E–G) SEM images show the presence of the extended filopodia-like structures that we termed tentacles. (H–K) Negative staining TEM images of thin sections through C. merolae tentacles show the presence of long filamentous structures that are oriented in a parallel fashion in the tentacle shaft (H–I) and in concentric circles in the tentacle head (J–K).