Table 4.
Phosphorylation sites of kinases and phosphatases showing different phosphorylation levels in the comparison of F10–12 to F1–9 and OF to F10–12.
| Family | Accession | Protein | Peptide (p-Site) | Location | F10–12/F1–9 | OF/F10–12 | TAIR Description |
|---|---|---|---|---|---|---|---|
| CDK | AT1G67580.1 | CDKG2 | MVKpSPDPLEEQR | — | −0.87/0.04 | 0/0.14 | Protein kinase superfamily protein |
| CDPK | AT4G23650.1 | CDPK6 | (CDPK6_1) RGpSSGSGTVGSSGSGTGGSR | — | −0.67/- | 0/- | Encodes calcium dependent protein kinase 3 |
| (CDPK6_2) RGSpSGSGTVGSSGSGTGGSR | — | −0.63/0.01 | 0/0.04 | ||||
| LRR_3 | AT2G26730.1 | F18A8.10 | (F18A8.10_2) GSEGQTPPGEpSRTPPRSVTP | — | −0.84/- | 0/- | Leucine-rich repeat protein kinase family protein |
| (F18A8.10_3) GSEGQpTPPGESR | — | −0.86/- | 0/- | ||||
| (F18A8.10_5) GSEGQTPPGESRpTPPR | — | −0.85/0.07 | 0/0.50 | ||||
| LRR_3 | AT3G17840.1 | RLK902 | SYVNEYpSPSAVK | — | −0.69/0.01 | 0/0.05 | Encodes a receptor-like kinase |
| MAP2K | AT4G29810.1 | MKK2 | FLTQSGpTFK | — | 0 | −0.62/- | Encodes a MAP kinase kinase 2 that regulates MPK6 and MPK4 |
| MAP3K | AT1G63700.1 | YDA | AEATVpSPGSR | — | −0.71/0.15 | 0/0.21 | Member of MEKK subfamily |
| MAP3K | AT2G17700.1 | STY8 | VQIEpSGVMpTAETGTYR | — | −0.72/0.06 | 0/0.02 | ACT-like protein tyrosine kinase family protein |
| MAPK | AT2G01450.1 | MPK17 | LEEHNDDEEEHNpSPPHQR | — | −0.78/0.01 | 0/0.24 | MPK17 Map kinase family member |
| MAPK | AT2G43790.1 | MPK6 | VTSESDFMpTEpYVVTR | S_TKc | −0.82/- | 0/- | Encodes a MAP kinase |
| MAPK | AT5G19010.1 | MPK16 | VAFNDTPTAIFWpTDpYVATR | S_TKc | 0/- | −0.78/- | Member of MAP Kinase |
| phosphatase | AT3G55270.1 | MKP1 | GVNTFLQPpSPNRK | — | 0/- | −0.69/- | Encodes MAP kinase phosphatase 1 |
| SLK | AT5G26751.1 | SK 11 | GEPNIpSpYICSR | S_TKc | −0.91/0.04 | 0/0.31 | Encodes a SHAGGY-related kinase involved in meristem organization |
Note: Values in each stage mean Log2fold change/P-value. The fold change represents the ratio of phosphorylation level of the detected peptide in a certain flower development stage to its phosphorylation level in another flower development stage. F1–9 means stage 1–9 flower buds, F10–12 means stage 10–12 floral buds, OF means opened flowers, and CL means cauline leaves. The domains in which each peptide is located are shown. PAS indicates the Per-ARNT-Sim (PAS) domain; S-TKc indicates the serine/threonine-protein-kinases catalytic domain; “—” indicates that the peptide was not in a specific domain.