Table 3.
Protein levels predicted by MS analysis, mRNA expression by real time quantitative reverse transcription polymerase chain reaction (qRT-PCR), and protein levels detected by Western blot analysis. − means un-detected; + means detected.
| Gene Symbol | Protein Name | Protein Expression Levels Determined by MS | mRNA Levels Determined by qRT-PCR | Protein Levels Determined by Western Blot | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| WJ-MSCs | NFFs | ASFs | WJ-MSCs | NFFs | ASFs | WJ-MSCs | NFFs | ASFs | ||
| EPHA2 | EPH receptor A2 | + | − | − | +++ | + | − | +++ | + | + |
| SLC25A4 | ADP/ATP translocase 1 | + | − | − | ++ | − | − | +++ | − | − |
| TLN2 | Talin2 | + | − | + | − | + | +++ | − | − | +++ |
| LMF2 | Lipase maturation factor 2 | − | + | + | − | ++ | ++ | + | + | ++ |
| ITGA2 | CD49b/Integrin subunit alpha 2 | + | + | − | ++ | + | − | +++ | ++ | − |
| VDAC3 | Voltage-dependent anion channel 3 | + | − | − | + | + | + | +++ | + | +++ |
| SOD2 | Superoxide desmutase | + | − | + | + | − | − | +++ | − | − |
| CDH2 | CD325/N-Cadherin | − | + | − | − | +++ | − | |||
| ITGA5 | CD49e/Integrin subunit alpha 5 | ++ | + | + | ++ | + | + | ++ | + | + |
| IGF2BP3 | Insulin-like growth factor 2 mRNA binding protein 3 | − | − | + | ++ | + | ++ | |||
| PLEC | Plectin | − | + | + | + | + | + | ++ | ++ | ++ |
| CLE7 | RNA transcription, translation and transport factor | + | − | − | + | − | − | +++ | + | + |
| CDH5 | CD144/VE-cadherin | ++ | + | − | ++ | + | + | |||
| NEXN | Nexilin | + | − | − | ++ | + | − | + | + | + |
| MLCK1 | Myosin light chain kinase | + | − | − | ||||||