Figure 1.

Acceptance of diet, and body weight development of 5xFAD mice fed with a standardized diet containing no, high or low amounts of wheat amylase trypsin inhibitors (ATIs) on a gluten-containing or gluten-free background. (a) Schematic representation of the diets and experimental regime. Mice were aged 4 weeks when all animals received the diet without gluten or ATI for 4 weeks to achieve a basal condition. Afterwards, animals were assigned to the four different diets for 8 weeks. Animals were sacrificed at a final age of 16 weeks. (b) Food consumption per day in the start phase (week 1–4) and on the different diets. (c) Body weight at sacrifice (n ≥ 8 per group; all data presented as mean + SEM; One Way ANOVA with Holm–Sidak’s multiple comparisons test; *, p < 0.05, **, p < 0.01).