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. 2020 Aug 27;21(17):6183. doi: 10.3390/ijms21176183

Figure 4.

Figure 4

Ectopic over-expression of miR-193a arms reduces Akt and Ekt pathways activation. Protein expression levels of B-Raf, p-Akt, p-Erk 1/2, total Erk 1/2, and β-actin after ectopic over-expression of miR-193a-3p and miR-193a-5p in melanoma cells. Protein expression of B-Raf, p-Akt, p-Erk 1/2, total Erk 1/2, and β-actin in A375 (A), MeWo (B), and 501Mel (C) cell lines after 72 h of transfection with miRNA mimics was performed by Western blot analysis. Data are representative of three independent experiments ± SD performed in triplicate. One-way ANOVA followed by Dunnett’s multiple comparisons test was performed. * p < 0.05, ** p < 0.01, *** p < 0.001, compared to corresponding control.