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. 2020 Aug 27;21(17):6190. doi: 10.3390/ijms21176190

Figure 1.

Figure 1

The conditional Cu(II) binding constant cK7.4 = [Cu(GHK)2]/([Cu(GHK)][GHK]). (A) UV-vis, (B) circular dichroism (CD), and (C) electron paramagnetic resonance (EPR) spectra of Cu/GHK 0.5:n (0.63 ≤ n ≤ 30 mM) at pH 7.4, 25 °C. (D) Speciation diagram obtained by decomposition of all UV-vis (diamonds), CD (squares), and EPR (circles) spectra (Figures S4–S6). The solid lines were calculated using the binding constant derived from (E) and log cK1 = [Cu(GHK)]/([GHK][Cu]) = 12.62 derived from potentiometry. (E) Determination of the binding constant cK7.4 using least squares regression. The margin of error derives from the 95% confidence interval (dashed lines).