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BRET recruitment assay. HEK293 cells were transfected with GαoA-RLuc, Gβ3, Gγ8-GFP2 and human KOR and treated with increasing concentrations of GL-I-30 or full agonist salvinorin A. Luminescence ratio data at 400 nm and 515 nm were normalized to vehicle and 10 nM salvinorin A. Non-linear regression was used to determined EC50 values. Data n = 24 is depicted as mean and SEM.
