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. 2020 Aug 22;25(17):3823. doi: 10.3390/molecules25173823

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Experimental protocol for investigating the chemopreventive ability of C3G-HB in NNK-induced lung tumorigenesis in A/JCr mice. NNK (100 mg/kg body weight/mouse) or saline was injected three weeks after the adaptation period; n = 5 in saline-injected groups, naive and control, and n = 10 in NNK-injected groups (no-C3G-HB, pre-C3G-HB, conti.-C3G-HB and post-C3G-HB). No-C3G-HB, not given C3G-HB supplemented diet; Pre-C3G-HB, C3G-HB supplemented diet only before NNK injection; Conti.-C3G-HB, C3G-HB supplemented diet continuously before and after NNK injection; Post-C3G-HB, C3G-HB supplemented diet only after NNK injection.