Figure 3.

Effect of CpG methylation in MT1 promoter on Lucia luciferase activity. Mouse embryonic fibroblasts (MEFs) were transfected with unmethylated or M.SssI-methylated Lucia reporter vectors carrying (A) deletions or (B) point mutations. Luciferase reporter vector (pGL4.12-SV40) was co-transfected for normalization of transfection efficiency. Reporter gene expression was measured using the Dual-Luciferase Reporter Assay System, and Lucia (luc) activity was normalized to firefly luciferase activity. Values are expressed as the means ± SD from three independent experiments. * p < 0.05, ** p < 0.01 versus the unmethylated vector group.