Figure 5.

Effect of CpG methylation on MT1 promoter activity in MTF1 KO cells. (A) Cells were transfected with unmethylated Lucia reporter vectors. Reporter gene expression was measured 6 h after Zn or Cd addition. Values for neither treatment group were significantly different from the untreated group. (B) MTF1-KO cells were transfected with unmethylated or M.SssI- or HhaI methyltransferase-methylated Lucia reporter vectors. Values are expressed as the means ± SD from three independent experiments. * p < 0.01 versus the unmethylated group. ^† p < 0.01 versus the M.SssI-methylated group.