Figure 5.

Subcellular localisation of Kv channel interacting protein (KChIP) subunit KChIP4 in the cerebellum. Electron micrographs showing immunoparticles for KChIP4 in the cerebellar cortex, as detected using the pre-embedding immunogold technique. (A–C) In the molecular layer, KChIP4 immunoparticles were detected in Purkinje cells, distributed along the extrasynaptic plasma membrane (arrows) of dendritic shafts (Den) and dendritic spines (s), as well as at intracellular sites (crossed arrows). (D–H) In the granule cell layer, immunoparticles for KChIP4 were mostly distributed along the somatic plasma membrane (arrows) and dendrites (Den) of granule cells (GC), as well as at intracellular sites (crossed arrows). At presynaptic sites, immunoparticles for KChIP4 were found along the plasma membrane (arrowheads) and intracellular sites (double arrowheads) of mossy fibre axon terminals (mf). Scale bars: (A–C,E–H), 250 nm; (D), 500 nm.