Figure 7.

Subcellular distribution of voltage-gated potassium (Kv) channel subunits Kv4.2 and Kv4.3 channels. Immunoreactivity for Kv4.2 and Kv4.3 in the cerebellar cortex as demonstrated by pre-embedding immunogold labelling. (A–D) Immunoparticles for both Kv4.2 and Kv4.3 in the molecular layer were found in the plasma membrane (arrows) and intracellular sites (crossed arrows) of PC dendrites (Den) and spines (s) establishing synapses with parallel fibres (pf). Although, at low frequency, Kv4.3 immunoparticles were also found in the plasma membrane (arrowheads) of parallel fibres (pf). (E–J) Immunoparticles for both Kv4.2 and Kv4.3 in the granule cell layer were found in the plasma membrane (arrows) of GC somata and GC dendrites (Den) in cerebellar glomeruli. In addition, immunolabelling was found intracellularly (crossed arrows). Few immunoparticles were also observed presynaptically along the plasma membrane (arrowheads) and intracellular sites (double arrowheads) in mossy fibre (mf) axon terminals. Scale bars: (A–J), 200 nm.