Table 4.
Components identified from the SC-CO2 extracts of Rh. sichotense and Rh. adamsii.
| No. | Identification | Formula | Calculated Mass | Observed Mass [M − H]− | Observed Mass [M + H]+ | Observed Mass [M + Na]+ | MS/MS Stage 2 Fragmentation | MS/MS Stage 3 Fragmentation | MS/MS Stage 4 Fragmentation | Species of Rhododendron |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Lepalol [5-(3-Furyl)-2-methyl-1-penten-3-ol | C10H14O2 | 166.217 | 165.06 | 147.01 | Rh. adamsii | ||||
| 2 | Caffeic acid [(2E)-3-(3,4-Dihydroxyphenyl) acrylic acid] | C9H8O4 | 180.1574 | 181.08 | 163.03; 135.11 | Rh. adamsii | ||||
| 3 | Azelaic acid [Nonanedioic acid] | C9H16O4 | 188.2209 | 210.09 | 192.12 | 175.06; 136.12 | Rh. adamsii | |||
| 4 | Calamenene [Cis-Calamenene] | C15H22 | 202.3352 | 203.09 | 147.05 | 119.06 | Rh. sichotense | |||
| 5 | Germacron | C15H22O | 218.3346 | 219.06 | 201.07; 149.07 | 159.07 | Rh. adamsii | |||
| 6 | Myristic acid (Tetradecanoic acid; N-Tetradecanoic acid) | C14H28O2 | 228.3709 | 251.09 | 150.48 | 149.08 | Rh. adamsii | |||
| 7 | Pentadecanoic acid (Pentadecylic acid) | C15H30O2 | 242.3975 | 243.06 | 201.01; 137.05 | 181.05; 135.04 | Rh. adamsii | |||
| 8 | Palmitoleic acid | C16H30O2 | 254.4082 | 277.09 | 275.04; 207.05 | 256.99 | 157.11 | Rh. adamsii | ||
| 9 | Cis-cyclopropan-9,10-hexadecanoic acid | C17H32O2 | 268.4348 | 269.02 | 185.97; 121.08 | 176.96 | 154.98 | Rh. adamsii | ||
| 10 | Linoleic acid [Linolic acid; Telfairic acid] | C18H32O2 | 280.4455 | 303.06 | 285.05; 163.00 | 180.95; 135.06 | 162.99 | Rh. adamsii | ||
| 11 | Stearic acid [Octadecanoic acid; Stearophanic acid] | C18H36O2 | 284.4772 | 285.07 | 284.18; 229.07; 163.02 | 180.90; 135.05 | 163.03 | Rh. adamsii; Rh. sichotense | ||
| 12 | Kaempferol [3,5,7-Trihydroxy-2-(4-hydro- xyphenyl)-4-H-chromen-4-one] | C15H10O6 | 286.2363 | 287.00 | 286.24; 204.96; 163.02 | 181.02 | 162.88 | Rh. adamsii | ||
| 13 | Cis-cyclopropan-9,10-octadecanoic acid | C19H32O2 | 292.4562 | 293.05 | 274.98 | 256.99; 162.98 | 201.03 | Rh. adamsii | ||
| 14 | Nonadecanoic acid [N-Nonadecanoic acid] | C19H38O2 | 298.5038 | 300.09 | 243.04 | 201.02 | Rh. adamsii; Rh. sichotense | |||
| 15 | Kaempferol 5-methyl ether | C16H12O6 | 300.2629 | 300.98 | 283.01; 177.01 | 264.98 | 200.98 | Rh. adamsii; Rh. sichotense | ||
| 16 | Farrerol [5,7-Dihydroxy-2-(4-hydroxyphenyl)-6,8-dimethylchroman-4-one] | C17H16O5 | 300.3059 | 301.05 | 283.04 | 241.01; 162.96 | Rh. adamsii; Rh. sichotense | |||
| 17 | Quercetin [2-(3,4-Dihydroxyphenyl)-3,5,7-trihy- droxy-4-H-chromen-4-one] | C15H12O7 | 302.2357 | 301.09 | 303.08 | 285.01; 163.02 | 180.97; 145.00 | 162.98 | Rh. adamsii; Rh. sichotense | |
| 18 | Dihydroquercetin [Taxifolin; Taxifoliol] | C15H12O7 | 304.2516 | 303.09 | 285.04 | 266.96; 241.09; 215.05; 135.05 | 171.02 | Rh. adamsii | ||
| 19 | Cannabigerorcinic acid [Cannabigerorcinolic acid; Cannabiorcogerolic acid] | C18H24O4 | 304.3808 | 303.08 | 285.05 | 241.07; 159.07 | 159.01 | Rh. adamsii | ||
| 20 | Docosane | C22H46 | 310.6006 | 311.10 | 293.06; 167.01 | 259.03 | 240.97; 162.96 | Rh. adamsii | ||
| 21 | 8-Demethyleucalyptin [5-Hydroxy-4′,7-dimetoxy-6-methylflavone; Pabalate; Sodium salicylate] | C18H16O5 | 312.3166 | 311.14 | 311.10; 182.99 | Rh. adamsii | ||||
| 22 | Arachic acid [Arachidic acid; eicosanoic acid] | C20H40O2 | 312.5304 | 311.14 | 335.04 | 303.06; 195.01 | 284.99; 238.14; 163.00 | 180.89; 135.14 | Rh. adamsii | |
| 23 | Azaleatin [5-O-Methylquercetin] | C16H12O7 | 316.2623 | 315.08 | 297.01; 167.04 | 235.04; 149.00 | Rh. adamsii | |||
| 24 | Myricetin [3,5,7-Trihydroxy-2-(3,4,5-Trihydroxyphenyl)-4H-Chromen-4-One] | C15H10O8 | 318.2351 | 317.08 | 299.01; 241.01 | 240.06; 197.09 | 238.99; 197.04 | Rh. adamsii; Rh. sichotense | ||
| 25 | Gossypetin [Articulatidin; Equisporol] | C15H10O8 | 318.2351 | 319.07 | 287.09; 176.98 | 146.99 | Rh. adamsii | |||
| 26 | Ampelopsin [Dihydromyricetin; Ampeloptin] | C15H12O8 | 320.251 | 319.08 | 317.01; 275.09 | 257.12; 217.11 | Rh. adamsii | |||
| 27 | Heneicosanoic acid [Heneicosylic acid] | C21H42O2 | 326.557 | 325.11 | 327.08 | 271.01; 217.03; 177.06 | 149.10 | Rh. adamsii; Rh. sichotense | ||
| 28 | Myricetin 5-Methyl ether [5-O-Methylmyricetin] | C16H12O8 | 332.2617 | 331.03 | 168.94 | 149.96 | Rh. sichotense | |||
| 29 | Esculin [Aesculin; Esculoside; Polichrome] | C15H16O9 | 340.2821 | 341.09 | 281.01; 217.11; 151.06 | 174.96 | Rh. adamsii; Rh. sichotense | |||
| 30 | Behenic acid [Docosanoic acid] | C22H44O2 | 340.5836 | 341.05 | 323.10; 243.11; 177.04 | 159.05 | Rh. adamsii; Rh. sichotense | |||
| 31 | Pentacosane (N-Pentacosane) | C25H52 | 352.6854 | 353.12 | 270.97; 162.97 | 180.93 | 162.96 | Rh. sichotense | ||
| 32 | Chlorogenic acid | C16H18O9 | 354.3087 | 355.09 | 287.05; 164.02 | 180.95 | 163.03 | Rh. adamsii; Rh. sichotense | ||
| 33 | Scopolin [Scopoloside; Scopoletin-7-glucoside; Murrayin] | C16H18O9 | 354.3087 | 355.02 | 323.00 | 303.96; 184.89 | 162.86 | Rh. adamsii; Rh. sichotense | ||
| 34 | Tricosanoic acid [N-Tricosanoic acid] | C23H46O2 | 354.6101 | 355.08 | 322.96; 163.00 | 180.96 | 162.96 | Rh. adamsii | ||
| 35 | Lignoceric acid [Tetracosanoic acid] | C24H48O2 | 368.6367 | 367.12 | 369.08 | 351.08; 285.02; 218.92; 162.98 | 163.02 | 144.97 | Rh. adamsii; Rh. sichotense | |
| 36 | Fraxin (Fraxetin-8-O-glucoside) | C16H18O10 | 370.3081 | 371.08 | 338.99 | 320.96; 177.03 | 224.96 | Rh. adamsii; Rh. sichotense | ||
| 37 | Daurichromenic acid | C23H30O4 | 370.4819 | 371.09 | 352.98; 287.08; 235.08; 179.02 | 231.04; 205.05; 162.99 | 180.93; 144.97 | Rh. adamsii; Rh. sichotense | ||
| 38 | Pentacosanoic acid [N-Pentacosanoic acid] | C25H50O2 | 382.6633 | 383.07 | 405.08 | 351.04; 287.99 | 229.04 | 211.03 | Rh. adamsii; Rh. sichotense | |
| 39 | Fraxetin-7-O-beta-glucuronide | C16H16O11 | 384.2916 | 383.09 | 365.09; 190.96 | 266.97; 215.02 | 170.97 | Rh. adamsii; Rh. sichotense | ||
| 40 | Beta-Sitosterin [Beta-Sitosterol] | C29H50O | 414.7067 | 415.04 | 384.02 | 369.01 | 338.00 | Rh. adamsii; Rh. sichotense | ||
| 41 | Cyanidin-3-alpfa-l-arabinoside | C20H19O10 | 419.3589 | 418.51 | 399.05; 319.02; 194.99 | 381.068 162.02 | 337.02; 253.08 | Rh. adamsii | ||
| 42 | Montanic acid [Octacosanoic acid] | C28H56O2 | 424.743 | 425.02 | 407.00 | 389.00; 348.98; 298.99; 240.97 | 333.00; 280.97; 173.02 | Rh. adamsii; Rh. sichotense | ||
| 43 | Alpha-Amyrin [Viminalol] | C30H50O | 426.7174 | 427.05 | 408.27; 308.99; 202.91 | 389.02; 309.01 | 373.08; 229.10; 142.80 | Rh. adamsii; Rh. sichotense | ||
| 44 | Lupeol [Fagarasterol; Clerodol; Monogynol B; Lupenol] | C30H50O | 426.7174 | 427.04 | 409.01; 202.99 | 389.02; 247.99 | 370.96; 264.80 | Rh. adamsii | ||
| 45 | Dihydroquercetin 3-arabinofuranoside | C20H16O11 | 432.3344 | 433.97 | 352.95 | 323.53; 271.96 | 241.95; 181.87 | Rh. adamsii | ||
| 46 | Afzelin [ Kaempferol-3-Rhamnoside; Kaempferin] | C21H20O10 | 432.3775 | 431.04 | 413.00; 372.98; 216.94 | 354.95; 167.01 | 336.98; 148.91 | Rh. sichotense | ||
| 47 | Quercetin-3-O-beta-xyloside (Reynoutrin; Quercetin 3-O-Beta-d-Xylopyranoside) | C20H17O11 | 433.3424 | 434.90 | 302.94 | 256.92; 164.96 | 228.91; 159.11 | Rh. sichotense | ||
| 48 | Avicularin (Quercetin 3-Alpha-l-Arabinofuranoside; Avicularoside) | C20H18O11 | 434.3503 | 433.09 | 415.07; 335.01; 176.98 | 397.06; 190.99 | 353.07; 253.99 | Rh. adamsii; Rh. sichotense | ||
| 49 | Pentoside dihydroquercetin | 436 | 435.16 | 416.54; 300.99; 231.01 | 397.02; 205.96 | 361.11; 283.02; 188.80 | Rh. adamsii; Rh. sichotense | |||
| 50 | Erithrodiol [3beta-Erytrodiol] | C30H50O2 | 442.7168 | 441.12 | 425.06; 381.05; 300.03; 217.04 | 363.06; 246.02 | 319.08; 201.02 | Rh. adamsii | ||
| 51 | Uvaol | C30H50O2 | 442.7168 | 443.22 | 425.01; 233.07 | 407.02; 325.01 | 388.99; 231.11 | Rh. adamsii; Rh. sichotense | ||
| 52 | Quercitrin [Quercetin 3-l-Rhamnoside; Quercetrin] | C21H20O11 | 448.3769 | 448.89 | 370.95; 282.93 | 352.95; 176.98 | 334.90; 222.92; 176.97 | Rh. adamsii | ||
| 53 | Catechin-7-O-glucoside | C21H24O11 | 452.4087 | 453.17 | 435.15; 336.07; 209.06 | 417.16; 336.11; 226.12 | 209.09 | Rh. sichotense | ||
| 54 | Micromeric acid | C30H46O3 | 454.6844 | 455.05 | 408.98; 246.98 | 391.05; 287.96 | 250.96 | Rh. adamsii | ||
| 55 | Hyperoside (Quercetin 3-O- galactoside; Hyperin) | C21H20O12 | 464.3763 | 465.02 | 302.91 | 256.94; 190.87 | 228.96; 172.75 | Rh. sichotense | ||
| 56 | Quercetin 3-O-glucoside [Isoquercitrin] | C21H20O12 | 464.3763 | 465.08 | 447.00 | 386.96 | 369.12; 172 | Rh. sichotense | ||
| 57 | Alpha.-Tocopherol-Beta-d-Mannoside [Dihydro-2H-Chromen-6-YI Hexofuranoside] | C35H60O7 | 592.8467 | 593.11 | 533.08 | 461.10 | 433.11 | Rh. sichotense |