Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 29;21(17):6253. doi: 10.3390/ijms21176253

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

LCN2 mAb attenuated the induction of chemokines and cytokines after tMCAo. We analyzed total RNA isolated from ipsilateral hemispheres of naive LCN2^+/+ and LCN2^−/− mice (+/+ con and −/− con), as well as at 23 h after tMCAo (+/+ tMCAo and −/− tMCAo), and LCN2^+/+ mice treated with LCN2 mAb at 4 h after tMCAo (+/+ tMCAo LCN2 mAb) using real-time RT-PCR (n = 6 per group). We compared differences in the relative mRNA expression of iNOS (A), IL-6 (B), CCL2 (C), and CCL9 (D) between the five groups using a one-way ANOVA and Newman–Keuls post hoc tests. The relative brain mRNA levels of iNOS, IL-6, CCL2, and CCL9 after tMCAo were significantly increased as compared with those in naive LCN2^+/+ mice. Additionally, the mRNA levels of iNOS, IL-6, CCL2, and CCL9 in LCN2^−/− mice or mice treated with LCN2 mAb were significantly reduced as compared with those in LCN2^+/+ mice after tMCAo (*** p < 0.001, ** p < 0.01, * p < 0.05).