Table 1.
Group | Number of Mice Used for Experiments | Mortality Rate | Number of Mice Used in the Figures |
---|---|---|---|
Figure 1 and Figure 2 | naive LCN2+/+: 3 | 0 | 3 |
(staining) | LCN2+/+ tMCAo: 3 | 0 | 3 |
Figure 3B,C | naive LCN2+/+: 3 | 0 | 3 |
(Immunoprecipitation–Western) | naive LCN2−/−: 3 | 0 | 3 |
LCN2+/+ tMCAo: 3 | 0 | 3 | |
LCN2−/− tMCAo: 3 | 0 | 3 | |
Figure 3E,F (ELISA) | LCN2+/+ tMCAo: 10 | 0 | 10 |
LCN2+/+ tMCAo-Ab: 10 | 1/10 (10.0%) | 9 | |
Figure 3D and Figure 6 | naive LCN2+/+: 6 | 0 | 6 |
(real-time RT-PCR) | naive LCN2−/−: 6 | 0 | 6 |
LCN2+/+ tMCAo: 8 | 2/8 (25.0%) | 6 | |
LCN2−/− tMCAo: 7 | 1/7 (14.3%) | 6 | |
LCN2+/+ tMCAo-Ab: 7 | 1/7 (14.3%) | 6 | |
Figure 4A–E (TTC staining) | LCN2+/+ tMCAo: 8 | 1/8 (12.5%) | 7 |
LCN2+/+ tMCAo-Ab: 8 | 1/8 (12.5%) | 7 | |
Figure 5A,B (blood–brain barrier (BBB)) | LCN2+/+ tMCAo: 6 | 1/6 (16.6%) | 5 |
LCN2+/+ tMCAo-Ab: 6 | 1/6 (16.6%) | 5 | |
Figure 5C–F | LCN2+/+ tMCAo: 4 | 0 | 4 |
(BBB–Western) | LCN2+/+ tMCAo-Ab: 4 | 0 | 4 |
Total | 108 | 9/108 (8.3%) | 99 |