Figure 5.
FoxP3 immunohistochemistry (IHC). (A) Coronal brain sections from sham-operated mice (left) or MCAO-mice (right) 24 h post-intervention detailing where the respective detailed images were taken from. (B) B1 refers to the contralateral cortical area, B2 refers to the contralateral white matter adjacent to the lateral ventricle, B3/4 refer to the ipsilateral equivalents to B1/2. (C) C1 refers to the contralateral cortical area, C2 refers to the contralateral white matter adjacent to the lateral ventricle, C3/4 refer to the ipsilateral equivalents to C1/2. Positive FoxP3 immunostaining was characterized by brown-colored cells with condensed nuclei and a subtle surrounding cytoplasmic area pertinent to lymphocytes, particularly in the peri-infarct cortex (C4) and the ipsilateral white matter (C3), which was not observed in sham-operated mice (B3 and B4, respectively). Original magnifications: (A) 2×; (B,C) 8×.