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. 2020 Aug 26;21(17):6143. doi: 10.3390/ijms21176143

Figure 3.

Figure 3

(A) BM cells from CVT (n = 8) and SPF (n = 8) mice at one month of co-housing were sorted to collect KL (Kit+Lin−) cells, pooled to form 4 KL cell pools for each housing condition, and then analyzed by single-cell RNA-seq. t-distributed stochastic neighbor embedding (tSNE) plot revealing KL single-cell gene expression in various hematopoietic stem and progenitor cell (HSPC) subsets in CVT and SPF mice: LTHSC, long-term hematopoietic stem cells; MPP, Multipotent progenitors; CMP, common myeloid progenitors; MEP, megakaryocytic-erythroid progenitors; GMP, granulocyte-monocyte progenitors; LMPP, lymphoid-primed multipotent progenitors. (B) Percentages of HSPC subsets in KL cells from CVT and SPF mice. (C) Gene expression heatmap and pathway analyses for LTHSCs showing up- and down-regulation of genes in CVT relative to SPF mice. (D) Gene expression heatmap and pathway analyses for GMPs showing up- and down-regulation of genes in CVT relative to SPF mice. * p < 0.05.