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. 2020 Aug 26;21(17):6143. doi: 10.3390/ijms21176143

Figure 5.

Figure 5

(A) B6 mice were maintained in SPF or were transferred to a conventional facility and co-housed (CVT) with mice from that facility for twelve months before they were euthanized and their BM cells being transplanted into lethally-irradiated (11 Gy) B6-CD45. 1 recipients in vivo through competitive repopulation. (B) Mice were bled at twelve months of co-housing in order to measure white blood cells (WBC), neutrophils (NEU), red blood cells (RBC) and platelets (PLT). The proportion and absolute number of blood CD11b myeloid cells were higher in CVT than in SPF mice. (C) BM cells from CVT and SPF donors were counted and analyzed to calculate recovery of total BM cells, KL (c-Kit+Lin−) cells, and KSL (c-Kit+Sca-1+Lin−) cells. BM cells from five CVT and five SPF donors were also cultured in vitro to measure colony forming cells (CFCs). No difference between CVT and SPF mice in these measurements. (D) Recipients of BM cells from CVT (n = 13) or SPF (n = 18) donors were bled and analyzed for donor contribution at six months following transplantation. (E) Recovery of total BM cells and donor-derived KSL cells in recipients of CVT and SPF donors at six months after BM transplantation. * p < 0.05; ** p < 0.01.