Figure A1.

(A) Revelation of differential gene expression in Lin⁻Kit⁺ (KL) cells from CVT and SPF mice by single-cell RNA-seq. BM cells from 8 CVT and 8 SPF mice were sorted into KL cells, pooled to form 4 cell pools for each housing condition, and analyzed by single-cell RNA-seq. Illustration of cell sorting, genomics, sequencing and data analyses. (B) Gene expression defines KL cells into various subsets: LTHSC, long-term hematopoietic stem cells; MPP, Multipotent progenitors; CMP, common myeloid progenitors; MEP, megakaryocytic-erythroid progenitors; GMP, granulocyte-monocyte progenitors; LMPP, lymphoid-primed multipotent progenitors. (C) Hematopoietic cell subsets distribution in KL cells from CVT and SPF mice based on single cell RNA-seq.