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. 2020 Aug 24;21(17):6089. doi: 10.3390/ijms21176089

Figure 3.

Figure 3

LSD1 knock-down reduces cell mobility and epithelial–mesenchymal transition (EMT)-related gene expression in kidney cancer cell lines. (A) The transwell infiltration assay of the same number of indicated shRNA-expressing Caki-2 cells was observed after two days of incubation. Cells that had migrated to the underside of the filters were fixed and photographed. Graph presents the invasion rate as the mean percentage of control. Error bars represent the mean ± SD of three independent experiments and * denotes p < 0.05 (Student’s t-test) versus ctr_sh group. (B) Western blotting of indicated EMT markers after 24 h treatment with indicated concentrations of epidermal growth factor (EGF) in control or LSD1 shRNA-expressing cells. (C) Quantitative PCR was used to examine the transcriptional level of the EMT marker genes in Caki-2 cells expressing control or LSD1 shRNA after two days of EGF treatment. Error bars represent the mean ± SD of three independent experiments and * denotes p < 0.05 (Student’s t-test) versus ctr_sh group.