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. 2020 Sep 2;21(17):6383. doi: 10.3390/ijms21176383

Figure 2.

Figure 2

Ionizing radiation (IR) increases protein expression and double-strand break (DSB) recruitment of USP14. (A) Representative confocal images and (B,C) quantification of USP14 IR-induced foci (IRIF) at the indicated time points in H460 and A549 cells following 10 Gy IR treatment. Nuclei were stained with DAPI. (D) Western blot analysis for USP14 and UCHL5 in H460 and A549 cells at the indicated times after IR treatment. β-actin was used as the loading control. (E) Representative confocal images following co-immunostaining to show co-localization of USP14 and γH2AX IR-induced foci (IRIF) in the indicated cell lines at 1 h following 5 Gy IR treatment. Nuclei were stained with DAPI. Data shown are the means ± SEM (n = 3).