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. 2020 Sep 2;21(17):6383. doi: 10.3390/ijms21176383

Figure 5.

Figure 5

Targeting USP14 inhibits homologous recombination. (A,B) Confocal immunostaining and graphical representation of RPA32 IRIFs in shControl (shCtrl)- vs. shUSP14-expressing H460 cells at 8 h following 5 Gy IR treatment. Nuclei were stained with DAPI. (C) Clonogenic survival analysis in H460 cells pretreated with 50 μM IU1 for 1 h +/− indicated doses of rucaparib (Ruc). Confocal immunostaining and graphical representation of IRIFs for (D,E) BRCA1 in H460 and (F,G) RPA32 in A549 cells at the indicated time points in shControl (shCtrl)- vs. shUSP14-expressing cells. Nuclei were stained with DAPI. Data shown are the means ± SEM (n = 3).